Embryogenic Callus Induction and Regeneration of Local Malaysian Rice (Oryza sativa L.) var. MR 219 and MR 253 from Mature Dehusked Seed Explants

Publication Date : 07/02/2018


Author(s) :

N.A. Muda, A. Shamsiah, N. A. Khirul Anuar.


Volume/Issue :
Volume 1
,
Issue 1
(02 - 2018)



Abstract :

The present study was carried out to develop protocol for optimum callus induction and regeneration of two local Malaysian rice varieties; MR 219 and MR 253 using mature dehusked seed explants. The frequency of callus induction was determined on MS medium supplemented with different concentrations of 2,4-D. Optimum callus induction frequency of rice var. MR 219 at 90% was recorded from the explants cultured on MS medium treated with 1.0 mg/L 2,4-D giving the optimum callus fresh weight of 0.33 ± 0.05 g after 4 weeks. Meanwhile, 70% callus induction frequency was recorded from the dehusked seed explants of rice variety MR 253 cultured on MS + 1.0 mg/L 2,4-D after 4 weeks. The optimum callus fresh weight was recorded at 0.28 ± 0.04 g. Clonal roots was observed


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